Selective Additive — Dry Form
A supplement for microbiological media enabling selective propagation of yeasts and fungi through bacteriostatic activity. Ascorbate based — free of chloramphenicol and other antimicrobial agents used in classic selective media.
Product description
A variant of the ZORIA-001 selective supplement, pre-weighed for TSB (tryptic soy broth) and other phosphate-buffered media such as LB. Enables selective cultivation of yeasts and filamentous fungi while limiting accompanying bacterial flora — free of chloramphenicol and other antibiotics.
The active substance is ascorbic acid and its salts in a proprietary composition with magnesium ions (patent application P.454716). It contains no calcium salts, so it is fully compatible with phosphate media (TSB, LB) — it forms no poorly soluble precipitate with phosphates.
For Sabouraud-type microbiological media use the ZORIA-001 (SAB) variant. A bacteriostatic, non-antibiotic mechanism — no selective pressure toward resistance. For research use only (RUO).
Identification
| Catalogue no. | ZORIA-001T |
|---|---|
| Form | Powder |
| Pack size | Pre-weighed for 1 L of TSB/LB medium |
| Yield | 1 L of prepared medium |
| Shipping packaging | Single-use pre-weighed sachet |
| Parcel weight | 0,05 kg |
Quantitative composition
| Ascorbic acid and its derivatives | Zastrzeżone / Proprietary |
| Magnesium salts | Zastrzeżone / Proprietary |
Preparation
- Dissolve the sachet contents in 100 ml of distilled or molecular-biology-grade water.
- Filter the solution through a 0.22 µm filter.
- Add to 900 ml of target medium at a temperature below 60 °C.
Scope of research applications
- Research selective cultivation of yeasts and filamentous fungi with suppression of accompanying bacterial flora.
- Work with species sensitive to classic antimicrobial agents (chloramphenicol).
- Comparative studies of selective media composition without selective pressure toward resistance.
Technical notes
- The active substance is heat-labile. Do not autoclave, boil or microwave solutions containing the additive. Add the working solution only to media cooled below 60 °C.
- The active substance is sensitive to light and oxygen. Store solutions protected from light, in containers filled as close to the cap as possible — the headspace air volume is the main factor accelerating degradation.
- A colour change to orange or brown indicates degradation of the active substance. Medium with a discoloured additive should be discarded.
- For spectrophotometric measurements, run a dynamic blank for each condition, due to possible absorbance drift from the formation of coloured degradation products.
- The additive is compatible with semi-solid and solid agar-containing media.
- The additive can cause a drop in pH by 1-2 units.
Safety note
The active substance is not classified as hazardous under the CLP Regulation and is widely used in the food and pharmaceutical industries.
- Ingestion: rinse the mouth with water.
- Eye contact: flush with plenty of water or saline.
- Skin contact: the working solution has a low pH and may irritate mucous membranes and damaged or inflamed skin. Healthy skin — rinse with water.
Storage and stability
| Storage conditions | Dry product: room temperature, dry, protected from light. Working solution — see stability table. |
|---|---|
| Shelf life | As per the date on the label |
| Stability after opening | Dry form stable under stated storage conditions |
| Stability after dilution | Temperature-dependent — see stability table |
| Period | Temperature |
|---|---|
| up to 4 days | ≤ 40 °C |
| up to 14 days | ≤ 25 °C |
| up to 28 days | ≤ 6 °C |
Lead time and delivery
| Lead time | up to 3 business days |
|---|---|
| Delivery | Parcel lockers, courier. Priority and international shipping — please contact us by e-mail. |
| Priority delivery | orders@zoriabiotech.com |
Batch and expiry
Documentation
Available on request · we will e-mail the PDF
Available on request · we will e-mail the PDF
Available on request · we will e-mail the PDF